You are here

Robert W. Hardy, PhD

Robert W. Hardy, PhD Professor
Department of Pathology
Phone: 205-934-9925


University of Waterloo, Canada BSc 1975 Biology & Chemistry
University of Toronto, Canada MSc & PhD 1982 & 1988 Clinical Biochemistry
Washington University, St. Louis, MO Post-doc 1988-90 Diabetes
Univ. of Alabama at Birmingham, Birmingham, AL Post-doc 1990-91 Diabetes

Research/Clinical Interest

Translational research
My primary area of interest is in clinical translational research involving oxidative stress and clinical chemistry. In particular I am interested in bringing assays for oxidative stress into the clinical lab. To this end I am participating in a project to establish a robust assay that describes mitochondrial bioenergetics.


  1. Shen M-C, Zhao X, Siegal GP, Desmond R, Hardy RW. Dietary Stearic Acid leads to a Reduction of Visceral Adipose Tissue in Athymic Nude Mice. PLoS One 2014, 9:e104083  4164353
  2. Zhao X, Rezonzew G, Wang D, Siegal GP, Hardy RW. Diet Modulation is an Effective Complementary Agent in Preventing and Treating Breast Cancer Lung Metastasis. Clinical & Experimental Metastasis 2014;31:625-638. 24832758 
  3. Chacko B, Kramer PA, Ravi1 S, Benavides GA, Mitchell T, Dranka BP, Ferrick D, Singa AK, Ballinger SW, Bailey SM, Hardy RW, Zhang J, Zhi D, Darley-Usmar VM. The Bioenergetic Health Index: A New Concept in Mitochondrial Translational Research. Clinical Science 2014;127:367-373.
  4. Chacko B, Kramer P, Ravi S, Hardy RW, Johnson M, Ballinger S, Darley-Usmar V. Methods for defining distinct bioenergetic profiles in platelets, lymphocytes, monocytes and neutrophils and the oxidative burst from human blood. Laboratory Investigation 2013;93:690-700. 3674307 
  5. Li C, Zhao X, Toline EC, Siegal GP, Evans LM, Ibrahim-Hashim A, Desmond RA, Hardy RW. Prevention of carcinogenesis and inhibition of breast cancer tumor burden by dietary stearate. Carcinogenesis. 2011;32:1251–1258. 3149204 
  6. Evans LM, Toline EC, Desmond R, Siegal GP, Ibrahim-Hashim A, Hardy RW. Dietary stearate reduces breast cancer metastasis burden in athymic nude mice. Clin Exp Metastasis. 2009;26:415-424.  2946234 
  7. Evans LM, Cowey SL, Siegal GP, Hardy RW. Stearate preferentially induces apoptosis in human breast cancer cells. Nutr Cancer. 2009;61:746–753.  2946230 
  8. Byon CH, Hardy RW, Ren C, Ponnazhagan S, Welch DR, McDonald JM, Chen Y. Free fatty acids enhance breast cancer cell migration through plasminogen activator inhibitor-1 and SMAD4. Lab Invest. 2009;89:1221–1228. 2905319 
  9. Deng Z-b, Poliakov A, Clements R, Liu C, Liu Y, Wang J, Xiang X, Garvey T, Michalek S, Grizzle WE, Hardy RW, Mobley J, Zhang H-G. Adipose tissue exosome-like vesicles mediate activation of macrophage induced insulin resistance. Diabetes. 2009;58:2498–2505. 2768161 
  10. Ji S, Hardy RW, Wood PA. Transgenic expression of n-3 fatty acid desaturase (fat-1) in C57/BL6 mice: effects on glucose homeostasis and body weight. J Cell Biochem. 2009;107:809-817.  2763528 
  11. Azrad M, Zhang K, Vollmer RT, Madden J, Polascik TJ, Snyder DC, Ruffin MT, Robertson CN, Moul JW, Brenner D, Hardy RW, Wendy Demark-Wahnefried W. Prostatic alpha-linolenic acid (ALA) is positively associated with aggressive prostate cancer: A relationship which may be modified by genetic variation in ALA metabolism PLoS One 2012;7:e53104. 23285256 
  12. Williams V, Martin R, Franklin JL, Hardy RW, Messina JL. Injury-Induced Insulin Resistance in Adipose Tissue. Biochemical and Biophysical Research Communications 2012;421:442-448. 22521887 
  13. Cowey S, Szafran AA, Kappes J, Zinn KR, Siegal GP, Desmond RA, Kim H, Evans L, Hardy RW. Breast cancer metastasis to bone: evaluation of bioluminescent imaging and microSPECT/CT for detecting bone metastasis in immunodeficient mice. Clin Exp Metastasis. 2007;24:389-401.  17541709 
  14. Cowey S, Hardy RW. The metabolic syndrome: a high risk state for cancer? Am J Pathol. 2006;169:1505-1522.  17071576 
  15. Zhao H, Hardy RW. Long chain saturated fatty acids induce annexin II translocation to detergent resistant membranes. Biochem J. 2004;381:463-469. 15099193